In this paper

the application of spectrum ratio to the segmentation of cell multi spectral micrograms is discussed and a new cell segmentation method is presented which combines spectrum information of multi spectral images with traditional segmentation methods. Firstly

background is removed from the multi spectral images. Secondly

two selected bandwidth images are divided to make a new ratio grey image. Thirdly

the new grey image is segmented with automatic multi threshold methods. Finally

the segmented regions are smoothed by morphological operations and the overlays of the plasm and the nucleus are obtained. In this paper

spectrum ratio technique is firstly introduced into the segmentation of cell multi spectral micrograms. The experiments with a diagnosis system on marrow cell images prove that this approach for segmenting cell images is rather accurate

fast

and robust to noise

and this method can be extended to the segmentation of other types of multi spectral micrograms.